Forhe Feiertage!
2024.12.20
PIPETTORS
PIPETTE TIPS
PIPET CONTROLLER
1. How should I work with my HTL pipettor?
It is important to handle your pipettors properly to maximize their longevity and maintain accurate readings.
It is recommended to:
2. What is pipettor maintenance?
Pipettors require regular cleaning, lubrication and calibration to maintain their performance over time.
3. What is pipettor calibration?
It is a test of your pipettor performance – to check whether the measurement results are within the permissible errors specified by the pipettor manufacturer on the basis of ISO 8655 standard. A calibration certificate listing the instrument’s accuracy and precision is issued for each pipettor manufactured at HTL.
4. Why do I need to have my pipettors calibrated and maintained?
Pipettor performance can change over time due to leakage, wear on parts and contamination. Cleaning and maintenance help eliminate these sources of incorrect dispensing. However, the accuracy and precision of your pipettors must be checked at regular intervals. Many quality systems and standards, such as GLP or GMP requirements and the ISO 9000 series, require that a regular, documented maintenance and calibration procedure be in place for all instruments. Regular pipettor calibration helps increase the quality and reliability of all procedures in which they are used.
5. How often do I have to calibrate my pipettor?
According to International Standard ISO 8655, pipettors need to be calibrated regularly, at least once a year or even more often, for example, every three to six months. The interval should be determined by the frequency of use, the types of liquids handled and the quality requirements of your particular application.
6. What does inaccuracy and imprecision mean?
7. What is ISO 8655?
International Standard ISO 8655 specifies the requirements for pipettors and pipettor calibration laboratories, providing detailed guidance for the respective environmental conditions, procedures and equipment. Pipettor calibration in accordance with ISO 8655 is performed using a repeatable and reliable measurement technology and equipment, such as balances, in a carefully controlled environment.
8. Can I calibrate my HTL pipettor myself?
Every HTL pipettor is delivered with a calibration tool to enable pipettor recalibration by the user. Detailed information on calibration procedures can be found in pipettor Instruction Manual.
9. How can I sterilize my pipettor?
10. Are HTL pipettors UV resistant?
HTL digital pipettors are UV resistant, which was confirmed by our tests. The recommended distance from the radiation source to exposed element should be not less than 50 cm. Prolonged and very intense UV exposure can cause de-coloration of pipettor parts, without affecting pipettor performance.
11. What is the warranty for HTL pipettors?
For warranty information please contact your local HTL distributor.
12. What do I do if my pipettor works improperly?
13. Is my pipettor resistant to chemicals such as…
HTL pipettors are manufactured with use of highly durable materials. While working, please consider chemical resistance of the following plastics: PEI, PP, PC, POM, PA, PPS, PVDF used to produce the pipettor parts.
14. How should I aspirate viscous and dense liquids?
Reverse pipetting is only possible with air displacement pipettors.
15. How should I set the volume of a variable volume pipettor?
The volume display shown by the counter has three digits, which should be read from top to bottom. In addition the lowest counter drum is printed in a scale which enables the setting of the volume in the minimum graduation range.
To set the required volume, use the lower section of the pipetting pushbutton or black adjustment knob. Discovery Comfort are equipped additionally with the locking system.
All volume setting procedures should end in the reduction of volume setting from a position just above the desired level to that level. As further examples:
16. Are the HTL pipettors ergonomic?
The design of HTL pipettors bases on decades of experience and reflects our deep understanding of end users needs and preferences. Within the years HTL representatives have paid hundreds of visits to all kinds of laboratories, on every continent in search for the most optimal characteristics of the manual liquid handling tools. This never ending research work of our dedicated distributors and our marketing crew allows us to find an ideal equilibrium between pipettor weight, materials applied, forces necessary for smooth but precise pipetting as well as the pipettor handle shape. Extensive pipetting is a hard physical work which in some extreme cases may cause Repetitive Strain Injuries (RSI). While crafting our pipettors we made sure that their properties give the user the sensation of comfortable work with the most popular pipettors on the market, but at the same time they minimize strain and potential risk of RSI.
The most important pro ergonomic features of HTL pipetting systems are:
The pipetting forces of all HTL pipettors belong to the lowest in the industry. Our research work of the last 3 years was aimed in particular at pipetting force reduction. The newest models of our pipettors feature low tension springs as well as smoothly running plunger sealing system resulting in effortless pushbutton operation at both pipetting and blowout stages.
HTL pipettors are renowned for their convenience and the sensation of the “familiar feel”. While the pipettor feels in the hand as the most popular pipettors on the market it offers wide finger support allowing for convenient hanging of the pipettor on the index finger and relaxed grip. The weight of the HTL pipettor gives the laboratory professional an optimal balance between low strain and secure pipettor handling. The diameters of the pushbuttons, together with their smoothly shaped edges ensure comfortable handling.
HTL pipettors are equipped with a number of systems aimed at reducing the force of tip ejection. The revolutionary system applied by HTL for reduction of the tip sealing and ejection force bases on the concept of retrievable shafts and cones. During the sealing of the tip on the pipettor the pipettor is being pressed against the rack of the tips. When the pressing force applied to the pipettor is sufficient for secure sealing the shaft starts to retrieve inside of the pipettor body, preventing tip jamming. Thanks to this unique, patented system the tip is being sealed with the minimal force and the force necessary for tip ejection is respectively small. The system of retrievable shafts is applied in all multi-channel pipettors. The system of precise setting of the tip ejector stroke in other models of HTL pipettors allows ejecting the tip with minimal effort and only after the Minimal movement of the thumb on the ejector pushbutton.
While designing the volume setting system in our variable volume single-channel and multi-channel pipettors we wanted to make sure that the location of our volume setting knob is proper for convenient one handle operation and that the knob rotates smoothly allowing for precise volume adjustment. Pipettor volume can be set precisely with one hand, by the turn of the volume setting knob or pushbutton. Additionally the volume setting knob of Discovery Comfort pipettors can be locked. This feature guarantees the reliability of results.
17. What parameters affect the accuracy of my pipettor?
HTL Pipettors are renowned for their outstanding accuracy and precision rates. What characterizes our equipment is the fact that it not only performs great when it’s brand new, but it also maintains its superbly reproducible performance after years of usage*. This important advantage is the result of both stringent quality control procedures applied by our QC Department and manufacturing know how gained by us within more than 45 years of pipettor manufacturing. From the very initial stages of the designing process, HTL pipettors are crafted to meet the requirement of long lasting reproducibility.
The following features of our pipettors allow us to attain this goal:
The properties of raw materials applied for the construction of HTL pistons combined with the unique technology of piston polishing result in extraordinary smoothness of this vital component. The smoother the move of the piston, the more repeatable its shape, the more accurate aspiration of the pipettor is.
In order to ensure the repeatability of the measurement, it is essential to keep the right proportion between the pipetting force, the blowout stop and the blowout force. On one hand it is essential to keep the operation forces at the lowest possible rate, on the other hoverer it is crucial to let the user feel the right moment when he should stop pressing the pushbutton down, before blowout. The tension of the pipetting and blowout springs applied in newest HTL pipettors is the result of decades of experience. Careful selection of the spring material and their shape allowed us to combine these two necessities in our pipettors. The new construction of the plunger assays applied in above pipettors allows for pipetting with unnoticeable effort, but to feel clear stop between pipetting and blowout at the same time. Thanks to this design the precision of work is easily obtained even by less experienced pipettor operators.
Extensive work with some pipettors may result in warming them up from the warmth the hand of the operator. The increase of pipettor temperature could cause an accuracy error due to expansion of the air drawing the liquid into the tip. Ultimately the warmer the air, the more expanded the air, the less liquid is aspirated and the larger the error. HTL pipettors resist this negative factor due to the low thermal conductivity of their handles. The material of our pipettor components coming in touch with operators’ palms do not worm up as fast as the plastic applied by most of other pipettor manufacturers.
In the newest design of HTL multi-channel pipettors our engineers made sure that the fact of possessing 8 or 12 shafts does not affect the reproducibility of the pipettor. The revolutionary solutions applied in multi-channels, ensuring identical aspiration in all multi-channel shafts are the alignment of the pistons and the retrievable shaft system. The alignment system guarantees that the plungers in all shafts move with the same paste regardless of the operator’s technique, while the retrievable shafts ensure that all tips are sealed securely without the risk of poor air tightness on the middle shafts.
18. Is my HTL pipettor durable?
HTL equipment is built to last. HTL pipettors base on well proven constructional concept of the most popular pipettors on the market. The application of solid, but light plastic handle combined with highest grade steel components (calibration screw, springs and low volume pistons) results in high mechanical and chemical durability of our products. There is no pipettor which would withstand all chemical and mechanical hazards, but the experience of our dedicated users show that in the most severe environment HTL pipettors shall last longer than plastic or aluminum products of most of other manufacturers.
Breaking shafts is the most common problem faced by all professionals working with the pipettors. When pipettor is dropped or swung during tip sealing, the breakage may occur. All versions of HTL pipettors are equipped with the shafts manufactured of PVDF. This space age material guarantees highest mechanical durability of the shaft and lowest durability deterioration under the influence of commonly used aggressive substances. In order to make sure that only shafts of the highest robustness parameters are assembled with our pipettors we introduced a frequent shaft durability test to the range of tests applied during our quality control procedure.
PIPETTE TIPS
1. How to disassemble and assemble the ejector and also how to adjust the length of the pipettor ejector?
Ejector assembly
1. Press the tip ejector button.
2. Place the tip ejector on the arbor.
3. Turn the plastic ejector bush counter-clockwise (left) until it’s latched.
Ejector adjustment
1. Remove the ejector pushbutton.
2. To increase the ejector length turn the plastic bush to the right.
3. To decrease the ejector length turn the plastic bush to the left.
Note: Some 10 μL tips may require a use of ejector cap, see the manual.
Ejector assembly
1. Remove the tip ejector button.
2. Use the screwdriver to turn the metal arbor clockwise to screw the ejector in and adjust its length.
Ejector adjustment
1. Remove the ejector pushbutton.
2. Use the screwdriver to increase or decrease the ejector length.
3. After ejector assembly or adjustment, put the ejector button back on its place.
Note: Some tips may require a use of ejector cap, see the instruction manual for details.
2. Can I use other manufacturers’ tips with HTL pipettors?
Although shafts of HTL pipettors are designed to fit all popular tips, the best accuracy and precision results are obtained with HTL tips. The performance of every pipettor is checked by gravimetric method and the results of test are printed in pipettor Quality Control Certificate.
Some other manufacturer’s tips do fit HTL pipettors, however we cannot assure that our stated performance specifications can be met when other tips are used.
3. How often do I need to change a tip?
Always change tips when you see droplets in the tip, when changing liquid or the tip has been damaged.
PIPET CONTROLLER
1. How can I sterilize my Swiftpet Pro?
The outer body of the Swiftpet Pro can be cleaning with isopropyl alcohol. Pipette holder, the nosepiece and micro filter are fully autoclavable at 120°C, 20 min.
2. Can I replace the battery of Swiftpet Pro?
Pipet controller Swiftpet Pro is delivered with 1 Li-ion battery, which is accessible by removing the battery cover and can be easily replaced. In case of long break of using the pipet controller, it’s highly recommended to remove the battery from the pipet controller or charge the battery every few months.
3. How long does it take to recharge the battery?
Full-charge time takes c.a. 3 hours. It allows for continuous work for 8 hours.
4. What is the warranty for HTL pipet controller?
For warranty information please contact your local distributor HTL.